INTRODUCTION TO BIOCHEMISTRY PRACTICAL

1. BIOCHEMISTRY
PRACTICAL
Presented By:
Ms. Sayali Dilip Powar
(M. Pharm )
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Content of Syllabus

3. Aim: Qualitative analysis of Carbohydrates
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Glucose
Fructose
Lactose
Maltose
Sucrose
 starch

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TEST OBJECTIVE
Molisch test To identify the carbohydrate from other
macromolecules lipids and proteins
Benedict's test Benedict's reagent is used as a test for the
presence of reducing sugars.
Barfoed’s Test to distinguish between reducing
monosaccharides, reducing disaccharides
and non reducing disaccharides.
Bial’s Test To distinguish between pentose
monosaccharide and hexose
monosaccharide
Seliwanoff's Test To distinguish between aldose and ketone
sugars

5. Aim: Identification tests for Proteins (albumin and
Casein
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Albumin
CASEIN

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TEST
1.Biuret test
2.Xanthoproteic Test
3.Millon’s Test
4.Ninhydrin Test