Genetic Fingerprinting

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Genetic fingerprinting ,[object Object],[object Object]

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How it is done – part 2 ,[object Object],[object Object],[object Object]

How it is done – part 2 ,[object Object],[object Object]

How it is done – part 3 ,[object Object]

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Effective disruption of the biological matrix (cell, tissue, environmental or biological sample) to release the nucleic acids. Denaturation of structural proteins associated with the nucleic acids (nucleoproteins) Inactivation of nucleases that will degrade the isolated product (RNase and/or DNase). Once the genomic DNA is bound to the silica membrane, the nucleic acid is washed with a salt/ethanol solution. These washes remove contaminating proteins, lipopolysaccharides and small RNAs to increase purity while keeping the DNA bound to the silica membrane column. There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries: disruption of the cellular structure to create a lysate, separation of the soluble DNA from cell debris and other insoluble material, binding the DNA of interest to a purification matrix, washing proteins and other contaminants away from the matrix and elution of the DNA.

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