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033 uptakes of spio by murine
1. Uptakes of SPIO by Murine
Macrophages and Monocytes
Daniel Chan
University of Colorado Health Sciences Center
12-19-2001
2. Synthesis of SPIO
• 1. SPIO coated with chondroitin sulfate
• 2 SPIO coated with standard dextran (6K)
• 3 SPIO coated with Amino-silane
• 4 SPIO coated with lipids
• 5 Lipid encapsulated SPIO coated with
amino-silane
3. Uptakes of SPIOs by murine peritoneal
macrophages and monocytes
• Normal mice were treated with mineral oil
by ip injection.
• 24 hours later, equal amount of various
SPIOs were injected intraperitoneally.
• 24 hours later, peritoneal macrophages and
monocytes were isolated.
• Uptakes of various SPIOs were examined
by light microscopy.
4. Uptake by Mouse Macrophages
SPIO coated with chondroitin sulfate
5.
6.
7.
8. Uptake by Mouse Macrophages
SPIO coated with standard Dextran
(6K)