SHAPE Society

1. Biodistribution ofBiodistribution of
SPIO in ApoESPIO in ApoE
Deficient MiceDeficient Mice
CNS-1-1d is MION madeCNS-1-1d is MION made
by Dr. Chanby Dr. Chan

2. 3 apoE deficint Mice
1mMol/kg SPIO injected intravenously
Sacrifice 2 hours, one day & two days after
injection
Staining of samples from aorta, heart, liver, lung,
Spleen and kidney with Perl’s, H&E, CD68

4. 1 Day After Injection,1 Day After Injection, Aortic RootAortic Root
H&E

5. 1 Day After Injection,1 Day After Injection, Aortic RootAortic Root
Perl’s

6. 1 Day After Injection,1 Day After Injection, Aortic RootAortic Root

7. 1 Day After Injection,1 Day After Injection, Ascending AortaAscending Aorta

8. 1 Day After Injection,1 Day After Injection, Ascending AortaAscending Aorta

9. Day3Day3 Aortic RootAortic Root

10. LiverLiver
Day2
Day3

11. Day3Day3 Descending aorta, H&EDescending aorta, H&E

12. Day3Day3 Descending aorta, no plaque no IronDescending aorta, no plaque no Iron

13.  We have different kinds of SPIO particles that weWe have different kinds of SPIO particles that we
are going to compare and see which one hasare going to compare and see which one has
optimal characteristics of macrophage uptake.optimal characteristics of macrophage uptake.
 We are doing macrophage uptake studies and ourWe are doing macrophage uptake studies and our
preliminary results show that these particles arepreliminary results show that these particles are
taken up by macrophagetaken up by macrophage