38. They assist immune response by :Inhibiting viral replication Activating the natural killer cells and macrophages Increasing antigen presentation to lymphocytes
40. Tissue infected by virus Interferon molecules produced It is released and diffused to the surrounding cells It binds to the receptor of the adjacent cells Production of anti – viral protein This protein blocks the viral replication
44. SEA (Staphylococcal Enterotoxin A) interferon inducer added (0.1 μl per ml) Mercaptoethanol added. Fetal calf serum added (10 %) Roller bottles were seeded with 300 ml cell suspension Roller bottles – gassed (7 % O2, 10% CO2, 83 % N – 1 min)
45. Suspension was rotated (8 rpm – 37 ⁰C , 4 days) Centrifuged (2000 rpm , 4 ⁰C) and supernatant collected Controlled core-glass beads were added to interferon supernatant. Glass beads containing INF was collected and centrifuged . Beads were washed with phosphate buffered saline.
46. Ethylene glycol added to elute interferon Removal of SEA inducer by Immunoabsorption Gel filtration was done on ultrogel to isolate the specific interferon. Fractions (5 ml) collected pH and temp. studies done to monitor heat stability and any residual antiviral activity.
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