in this PDF, we will discover: -introduction to RNA organic extraction - trizol method - uses and application - importance of extraction in the medical field

1. RNA organic extraction

2. guanidinium-acid-phenol method
• Guanidinium thiocyanate-phenol-chloroform extraction method is
often referred to as a conventional method of RNA isolation
• known as the Trizol method of RNA isolation (Guanidinium
thiocyanate and phenol mixture is popularly known as Trizol reagent)
• the sample is treated with :
❑guanidinium isothiocyanate
❑ acidic phenol
❑ chloroform

13. Advantages Disadvantages
High yield of RNA Compared to
other RNA isolation protocols
• Time consuming
This method is particularly
advantageous in situations
where cells or tissues are
enriched for endogenous
RNases
• Many harmful chemicals compared
to column based methods

14. Biochemical and molecular techniques
qRT-PCR
cDNA library
construction
northern
blotting
transcriptome
studies
array analysis

15. References
• https://pubmed.ncbi.nlm.nih.gov/20516177/