5. Cefaclor anaphylaxis in children Novembre Allergy 2009:64:1233 Amoxicillin at concentrations of 2 and 20 mg/ml in normal saline for both prick and intradermal tests , cefaclor at concentrations of 2 and 50 mg/ml in normal saline for prick testing , and 2 mg/ml for intradermal one. We prepared cefaclor sterile solutions no more than 2 h before testing, using the powder obtained from a capsule, as previously described. (Romano A, Clin Exp Allergy 2005;35:1234–1242) When prick tests were negative, 0.02 ml of the reagent solution was injected intradermally on volar forearm skin . Readings were made 20 min after injections. Results were considered positive when an increase larger than 3 mm in the initial wheal diameter accompanied by erythema was present.

9. Resting basophils do not express CD63 (anchored in the basophilic granule) and weakly express CD203c. The cross-linking of two FcεRI (induced by an allergen or anti-IgE antibodies) provokes the histamine release (and as a consequence the CD63 expression ) and the upregulation of CD203c. The rise in CD63 or CD203c expression (measured by fluorescence 2 / Phycoerythrin) before and after allergen challenge reflects thus the basophil activation / degranulation in response to an allergen. basophil activation test by flow cytometry * *

14. Immunogloblin E-mediated immediate allergic reactions to dipyrone: value of basophil activation test in the identification of patients. Gómez C EA 2010;39:1217 Background: Pyrazolones are a major cause of immediate IgE-mediated reactions to drugs in many countries. Objective: The aim of the study was to evaluate a group of patients with this type of reaction by basophil activation test (BAT), focusing on the influence of the time since the reaction on the rate of positive results.