BIOCHEMISTRY INHIBITORS OF ELECTRON TRANSPORT CHAIN AND OXIDATIVE PHOSPHORYLATION PREVIOUS AIIMS, NEET, PGI CHANDIGARH QUESTIONS , NEET QUESTIONS

1. Inhibitors of the
ETC proper
Inhibitors of
oxidative
phosphorylation
[FoF1ATPase]
Uncouplers of
oxidative
phosphorylation

3. » Complex I - NADH-Coenzyme Q reductase
» Complex II - Succinate-Coenzyme Q reductase
» Complex III - Cytochrome c reductase
» Complex IV - Cytochrome c oxidase
.

4. Complex-I
Complex-III
Complex-IV
Blocking
ETC Proton pumping
ATP synthesis
Oxygen uptake
Results
in block

5. NAD FMN Fe-S CoQ Cyt.b Fe-S Cyt.c1
Cyt.c Cyt.aa3 O2

6. Chlorpromazine
guanethidine
Antibiotic
Barbiturates
InseCticide
Rotenone
Amobarbitol
Piericidin

7. NAD FMN Fe-S CoQ Cyt.b Fe-S Cyt.c1
Cyt.c Cyt.aa3 O2
MALONATE TTFA CARBOXIN

8. NAD FMN Fe-S CoQ Cyt.b Fe-S Cyt.c1 Cyt.c Cyt.aa3 O2
Dimercaprol
Antimycin A
phenformin

9. NAD FMN Fe-S CoQ Cyt.b Fe-S Cyt.c1 Cyt.c Cyt.aa3 O2
Cyanide
CO
H2S
azide

10. Block Phosphorylation directly by
inhibiting FoF1ATPase
Oligomycin
Dicyclohexyl
carbodiimide
[DCCD]

13. Atractyloside
Bongregate secreted by
pseudomonas
Inhibitor of ADP entry/ATP exit

15. Uncouplers
1. Dinitrophenol amphipathic increases
permeability of imm to protons
2. Dicoumarol
3. Cccp chlorocarbonylcyanide phenyl hydrazone
4. Valinomycin streptomyces
5. Calcium

18. Physiological uncouplers
1.Excess thyroxine
2.EFA Defeciency
3.LongChain Fatty Acid IN Brown Adipose
Tissue
4.Unconjugated hyperbilirubinemia
5.Ucp /thermogen in brown fat

19. THERMOGENIN
a natural
uncoupler
H+

21. Inner mitochondrial membrane is not
permeable to cytoplasmic NADH

22. Malate – aspartate
shuttle
Glycerol phosphate
shuttle

23. Malate – aspartate shuttle

24. Glycerol phosphate shuttle

25. ENERGY CURRENCY OF CELL
ATP
ADP
ADP+Pi
AMP+Pi
AMP Adenosine+Pi
G0= -7.3Kcal/mole
G0= -7.3Kcal/mole
G0= 3.4Kcal/mole

27. Cyanide is toxic because it
A. Inhibits cytochrome oxidase
B. Forms cyan met Hb
C. Inhibits ATP carrier in mitochondria
D. Inhibits Na-K ATPase

28. A. Inhibition of ATP synthase
B. Inhibition of electron transport
C. Uncoupling of oxidation and
Phosphorylation
A. Accumulation of ATP
Dinitrophenol causes

29. A. Co
B. H2s
C. Cyanide
D. Amobarbital
E. Rotenone
Cytochrome oxidase I in oxidative
phosphorylation is inhibited by

30. A.Rotenone
B.Antimycin A
C.Cyanide
D.Actinomycin
The electron flow in cytochrome c
oxidase can be blocked by

33. A. One rotation produces 3ATP
B. Fo unit function as proton channel
C. α and β subunits rotate
D. Bent axle is formed by γ and ε subunits
True about ATP synthase is all except

36. A. oxidase
B. Hydrolase
C. Peroxidase
D. dehydrogenase
During phagocytosis the metabolic process called
respiratory burst involves the activation of

37. A.Adrenal gland
B.Skeletal muscle
C.Brown adipose tisue
D.heart
The specialized mammalian tissue in which fuel
oxidation serves not to produce ATP but to generate
heat is

38. A.Exergonic and anabolic
B.Exergonic and catabolic
C.Exergonic and catabolic
D.Cytochrome c reductase involved
Internal respiration are

39. Among the following the maximum
redox potential is for
A. NADH?NAD
B. Succinyl co A fumarate
C. Ubiquinone
D. Fe+

40. Cellular oxidation is inhibited by
A.Cyanide
B.Carbon dioxide
C.Choclate
D.Carbonated beverages

41. Cytochrome oxidase is
inhibited by
A. Cyanide
B. aluminium phosphate
C. Phenobarbitone
D. Carbon monoxide
E. NO

42. About oxidative phosphorylation true is
A.Genration of ATP
B.Generation of ADP
C.Utilization of ATP
D.Utilization of NADP

43. In oxidative phosphorylation the ATP
production and respiratory chain
are linked by
A. Chemical methods
B. Physical methods
C. Chemiosmotic methods
D. Conformational changes

44. Mitochondrial membrane protein
contain transporter of
A.NADH
B.Acetyl coa
C.NADPH
D.ATP

48. Cytosolic cytochrome c mediates
A. Apoptosis
B. Electron transport
C. Krebs cycle
D. glycolysis

49. Mallate shuttle is seen to occur in
A.Glycolysis
B.Glycogenolysis
C.HMP shunt
D.gluconeogenesis

51. During gluconeogenesis reducing
equivalents from mitochondria to cytosol
are transported by
A.Malate
B.aspartate
C.Glutamate
D.oxaloacetate