Integrated DNA Technologies

next generation sequencing qpcr dna sequencing crispr/cas9 crispr qpcr assays ngs oligonucleotides genome editing target enrichment dna ngs target enrichment alt-r™ crispr-cas9 system target capture genome sequencing gblocks gblocks gene fragments oligonucleotide synthesis gene expression cas9 dna synthesis synthetic biology pcr snps xgen blocking oligos rna genomics assay design umi adapters xgen® dual index umi adapters crispr genome editing ngs adapters nexgen xgen® target capture quantitative pcr qpcr troubleshooting gene synthesis exome sequencing acute myeloid leukemia genotyping ncbi xgen lockdown probes unique molecular identifiers rhamp rhamp snp genotyping system snp ngs analysis alt-r™ crispr-cpf1 system crispr-cpf1 cpf1 ribonucleoprotein cancer research ngs library preparation alt-r igem gene silencing sirna blast qpcr design multiplexing intercalating dyes gene assembly cancer panels acute myeloid leukemia cancer panel primers primer design antisense oligonucleotides rt-qpcr qpcr data analysis qpcr normalization amplification curve rtpcr grna umis crispr-cas12a ngs ctdna workflow ctdna circulating tumor dna snp genotyping gene disruption nickase experiments homology directed repair hdr cas9 nickases illumina dual index adapters smrt® sequencing pacific biosciences pacbio hybrid-capture technology alzheimer’s disease crispr-cas9 system single nucleotide polymorphisms genotype pcr genotyping multiplex sequencing index hopping dual-matched adapters gene knockout rnp complexes precision medicine molecular tagged adapters dna probes qpcr master mix real-time quantitative pcr qpcr mastermix ncbi tools national center for biotechnology information basic local alignment algorithm tool biological research blast tool ncbi blast high throughput qpcr lncrna knockdown lncrna small interfering rna antisense disassociation curves melt curve analysis melt curves target capture probes gene targeting genome modification clinical diagnostics hybridization probes hybridization enrichment oligo synthesis xgen® predesigned gene capture pools rnase h2-dependent pcr rhpcr dsirna rnai copy number variation assays cnv assays qpcr design strategies primetime® qpcr assays threshold cycle quantification cycle sybr melt curves problematic qpcr sybr green 5 nuclease assays oligoanalyzer oligos melting temperature tm nucleic acid synthesis restriction cloning cloning isothermal assembly topo cloning gene construction gene fragments antibodies biomarker detection biomarkers antibody-oligonucleotide conjugates antibody conjugates bioconjugation personalized medicine aml transposons scitools ultramers microrna mirna biogazelle rt-pcr